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Flow Cytometry Facility

Flow cytometry is a technology that simultaneously measures and then analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. The equipment can both analyse and sort single cells based on these characteristics and researchers utilize this service for applications in immunology, cell biology and microbiology.

The UNSW Mark Wainwright Analytical Centre flow cytometry facility provides comprehensive and technical cell analysis and sorting services to groups within the campus and the wider Sydney research basin. The facility currently incorporates one analysis instrument BD LSRFortessa™ and access to one cell-sorting instrument FACSAria™II (ugrade). Basic information on instruments, capabilities and configurations are given below as well as their respective links to company websites containing more detailed information. Cell sorting services are provided in both operator assisted and unassisted systems and charges are listed on the Pricing page.

For Cell Sorting requests please complete this online 'Cell Sorting Request Form'. All Cell Sorting bookings must be accompanied by the submission of this form. If you do not have any mail client such as 'Outlook' the form will not work and you will be required to submit an email with all necessary information included.

If you require clarification or have queries about specific applications, please contact Chris Brownlee.

Equipment Description

BD LSRFortessa™ SORP cell analyser (BD Biosciences, San Jose, USA)

The BD LSRFortessa™ SORP (special order research product) cell analyser system is equipped with five excitation lasers wavelengths (355, 405, 488, 561, 640 nm) and has the capacity of detecting up to 18 fluorescent and 3 scatter options of cells at speeds of up to 15,000 events per second.

A high throughput sampling (HTS) option allows users to analyse sample directly from a plate (12, 24, 96 and 384 wells). Included is a small particle detection option (SPO) and a high-powered 488nm laser allowing users to resolve particles in the ranges of 0.2um – 1.0um. Acquisition software is FACS™ Diva generating standard FCS3.0 files for third party software analysis packages.

Click here for configuration.

More information: http://www.bdbiosciences.com

BD™ High Throughput Sampler Option (HTS)

To improve experimental workflow, the optional BD™ High Throughput Sampler (HTS) provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates. In high-throughput mode, the HTS option can speed through a 96-well plate in fewer than 15 minutes with less than 0.5% sample carryover from one well to the next. Low carryover is essential in research applications to ensure sample purity and data integrity.

Fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample injection, and probe washing. Standard throughput mode can be selected for acquisition of larger sample volumes.

BD FACSAria™ II cell sorter (BD Biosciences, San Jose, USA)

The BD FACSAria II upgraded cell sorting system is equipped with three excitation lasers wavelengths (405, 488, 640 nm) and has the capacity of detecting up to 10 fluorescent, 2 scatter options and is capable of sorting between 4,000 and 20,00 cells per second. As add-ons it contains an Automated Cell Deposition Unit (ACDU) that allows sorting of single cells onto multiwall plates and slides. Acquisition and sorting software is FACS™ Diva generating standard FCS3.0 files for third party software analysis packages.

Click here for configuration.

More information: http://www.bdbiosciences.com

BD FACSCanto™ II Analyser (BD Biosciences, San Jose, USA)

BD FACSCanto™ II is equipped with three excitation lasers wavelengths (405, 488, 640 nm) and has the capacity of detecting up to 10 fluorescent, 2 scatter options and is capable of analyzing samples between 100 and 12,000 cells per second and software is FACS™ Diva generating standard FCS3.0 files for third party software analysis packages.

Click here for configuration.

More information: http://www.bdbiosciences.com

BD Influx™ Cell Sorter (BD Biosciences, Cytopeia, USA)

The BD Influx™ Cell Sorting system is equipped with five excitation laser wavelengths (355nm UV, 405nm, 488nm, 561nm and 642nm) and has a flexible configuration with a number of filter set options, detecting up to 24 channels (21 fluorescent channels, two forward scatter channels and one side scatter). With sorting speeds up to 70,000 cells per second this high-end Cell Sorter runs FACS Sort™ software, generating FCS3.0 files. Sorting collection options include 2, 4 and 6-way sorting, single cell into 96, 384 or 1536 well plates, slides, 5 ml or 15 ml tubes.

Class II Biosafety containment is provided by Contained Air Solutions (CAS)

Click here for configuration.

More information: http://www.bdbiosciences.com

BD FACS Jazz™ Cell Sorter - pre release model (BD Biosciences, Cytopeia, USA)

The BD FACS Jazz™ Cell Sorting system is equipped with two excitation laser wavelengths (488nm and 642nm) and has a flexible configuration with a number of filter set options, detecting up to 8 channels including forward and side scatter. With sorting speeds up to 10,000 cells per second this simplified Cell Sorter runs FACS Sort™ software, generating FCS3.0 files. Sorting collection options include 2 and 4-way sorting, single cell into 96 or 384 well plates, slides, 5 ml or 15 ml tubes.

Class II Biosafety containment is provided by Contained Air Solutions (CAS).

Click here for configuration.

Authorised by the Head of Biological Resources Imaging Laboratory, Mark Wainwright Analytical Centre
UNSW CRICOS Provider Code: 00098G
ABN: 57 195 873 179
Page Last Updated: Tuesday, January 15, 2013 11:27 AM